J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (10): -.
• Research Articles •
Zhang Hua-min, Huang Hai and Liu Yu
Abstract: The extract of wheat chloroplast membrane proteins was precipitated by different saturation of (NH4)2SO4. Pellet of 0–30% saturation showing high binding activity to 3H-6BA wax loaded on the affinity chromatography column which was prepared by coupling 6BA to epoxy activated sepharose 6B. The CTK-binding protein was eluted from the BA-sepharose 6B column with Tris buffer containing 0.1 mmol/L 6BA. It showed a single protein band on PAGE and the apparent molecular weight was about 250kD. Two bands with molecular weight of 60kD and 66kD were detected on SDS-PAGE. It was supposed that the protomer of CTK-binding protein was a tetramer of two subunits.
Key words: Wheat chloroplast, Cytokinin-binding protein, Affinity chromatography
Zhang Hua-min, Huang Hai and Liu Yu. Isolation and Purification of Cytokinin-Binding Protein from Wheat Chloroplasts[J]. J Integr Plant Biol., 1991, 33(10): -.
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