J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (6): -.

• Research Articles •    

Fluorescence Microscopic Observations on the Distribution Patterns of Actin Filaments in Stigma Cells of Eichhornia crassipes

Zhang Wei-cheng,Jin Shi-ping and Yan Wen-mei   

Abstract: The distribution patterns of actin filaments in the non-fixed stigma of Eichhornia crassipes (Mart) Solms were examined with fluorescence microscopy by using FITC-phalloidin as fluorescence probe. In the finger-like papillae the distribution patterns of actin filament varied greatly with actin localization. In the basal region fusiform bodies emitting intense fluorescence were scatteredly distributed. In the middle zone(often occupied by dense cytoplasm) a network composed of numerous actin filaments appeared. These filaments of various diameters lay more or less parallelly to the cell axis, extending upwards and gradually merging into some thick dense bundles . In the apical region a few actin filaments sparsely and longitudinally distrubuted in the subcortical cytoplasm,and diffuse fluorescence often appeared in the spheroidal protrusion. Furthermore,an actin network composed of very thin filaments in the periplasm of the cell was observed ;the constituent filaments were in helical arrangement and often branched and interconnected. Considering possible relationship between the actin configurations and the physiological activities and functions of the stigma cells, it is proposed that the active cytoplasmic streaming, the translocation of solutes towards the apical region ,the active secretion of exudate from the spheroidal protrusion and maintaining of the structural integrity and stability of periplasm, all these might be considered as certain physiological events being affected or regulated by the actin filament patterns described above.

Key words: Actin filaments, FITC-phalloidin, Eichhornia crassipes, Stigma

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