J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (1): 127-135.DOI: 10.1111/jipb.12304

• Research Articles • Previous Articles    

Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora

Yumi Hirakawa1, Toshihisa Nomura2, Seiichiro Hasezawa1 and Takumi Higaki1*   

  1. 1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha Kashiwa, Chiba, Japan
    2RIKEN, Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan
  • Received:2014-08-16 Accepted:2014-10-27 Published:2015-01-01
  • About author:*Correspondence: E-mail: higaki@k.u-tokyo.ac.jp

Abstract:

Vacuoles are suggested to play crucial roles in plant defense-related cell death. During programmed cell death, previous live cell imaging studies have observed vacuoles to become simpler in structure and have implicated this simplification as a prelude to the vacuole's rupture and consequent lysis of the plasma membrane. Here, we examined dynamics of the vacuole in cell cycle-synchronized tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells during cell death induced by application of culture filtrates of Erwinia carotovora. The filtrate induced death in about 90% of the cells by 24 h. Prior to cell death, vacuole shape simplified and endoplasmic actin filaments disassembled; however, the vacuoles did not rupture until after plasma membrane integrity was lost. Instead of facilitating rupture, the simplification of vacuole structure might play a role in the retrieval of membrane components needed for defense-related cell death.

 

Hirakawa Y, Nomura T, Hasezawa S, Higaki T (2015) Simplification of vacuole structure during plant cell death triggered by culturefiltrates of Erwinia carotovora. J Integr Plant Biol 57: 127–135. doi: 10.1111/jipb.12304

Key words: Actin filaments, defense-related cell death, Erwinia carotovora, tobacco BY-2 cells, vacuoles

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