J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (7): -.

• Research Articles •    

Somatic Embryogenesis and Plant Regeneration from Papaya Suspension Cultures

Ye Ke-nan, Ma Lei and Li Bao-jian   

Abstract: Compact embryogenetic calli were obtained from explants on P3 medium after 4 weeks of culture and high-frequency somatic embryogenesis occurred after these calli were transferred into suspension culture. Experimental data showed that low level (0.2%W/V) of activated charcoal had beneficial effects on somatic embryogenesis. Abundant calli on P4 medium however, showed no embryogenesis. On the other hand, callus induction and somatic embryogenesis varied with different rarities of exptants. The efficiency of somatic embryogenesis was much higher, if roots were used as explants, whereas stems were more suitable for callus formation Mature somatic embryos with cotyledons were cultured on MS medium containing different plant hormones. The optimum medium for germination and growth of entire plantlet was Mso medium. The somatic embryos on MS2, MS and MS3 media germinated rapidly, but formed excessive callus from the surface of germinating embryos.

Key words: Papaya (Carica papaya L.), Embryogenesis, Suspension culture, Plant Regeneration

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