J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (6): -.

• Research Articles •    

Suspension Culture of Endosperm Callus Cell and Amitosis In Vitro

Gu Shu-rong, Gui Yao-lin, Liu Shu-qiong and Xu Ting-yu   

Abstract: The endosperm calli were induced on MS basic medium supplemented with lppm 2,4-D, 0.5ppm KT and 5% sucrose. The medium which contained lppm BAP, 0.1ppm NAA and 2% sucrose was used for cell suspension culture. In suspension cell culture, amitosis of cleavage division of nucleus have been observed after 5 days of culture. First the nuclear membrane and nucleolus disappeared. The crevice appeared in the center of the nucleus, and the nucleus divided into two daughter nuclei of similar size and each with a nucleolus. The daughter nucleus resembled an eye in shape. Following the emergence of cell wall, the two new unequal cells were produced. Such amitotic division proceeded repeatedly until the callus developed and eventually plantlet regenerated.

Key words: Lycium, Endosperm callus, Cell suspension culture, Amitosis of cleavage division, Plantlets regeneration

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