J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (3): -.

• Research Articles •    

Purification and Characterization of the polygalacturo nase from Tomato Fruits

Shen Quan-guang, Liu Cun-de, Yan Tian, Ju Rong and Wang Fei-lan   

Abstract: The polygalacturonase (PG) isolated fron the pericarp of fully ripe tomato fruits was purified by (NH4)2SO4 precipitation, carboxyme-thylsepharose ion-exchang column and sephadex G-75 gel filtration. Specific activity of purified PG was 1.5 μmol galacturonic acid mg-1 protein min-1, which was 30 times as high as that of the crude extract with 1.7mol NaC1. When the elution separated by second sephadex G-75 column was analyzed by SDS-PAGE, only a single protein band was detected. It was shown by heat and pH experiments of the purified enzyme that the enzyme activity retained 50%, after treatment with heat at 50℃ for 10 min, and that the optimal pH was 4.6.

Key words: Tomatoes pericarp, Polygalacturonase

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