J Integr Plant Biol. ›› 1992, Vol. 34 ›› Issue (12): -.

• Research Articles •    

Cryopreservation of Calli of Arnebia euchroma

Li Guo-feng, Ye He-chun, Dong Jiao-wang, Jian Ling-cheng,Sun Long-hua and Gu Li-min   

Abstract: In this preliminary experiment two kinds of cryoprotectants were used: (1). 10% DM-SO + 0.5 mol/L sorbitol; (2). 7.5% DMSO +5% glycerin +5% sucrose. Calli samples were taken at day-10 and day-15 after subculture respectively. The specimens were cooled at a rate of l℃/min from 0℃, kept –40℃ for 2h, put into liquid nitrogen(–196℃) and then thawed in 40℃ water bath. Calli which bare been subcultured for 10 days showed normal growth by using cryoprotectant 2 and slow-freezing method mentioned above which indicated that they were effective methods for cryopreservation of Arnebia euchoma. Normal growth of the callus after cryopreservation could be restored, but the initial growth rate was somehow retarded. Using polycrylamide gel electrophoresis analysis no difference in esterase and peroxidase isozymes was found in both types of the treated calli. The present experiment indicates it is possible to use this slow-freezing method for germplasm cryopreservation of Arnebia euchroma.

Key words: Arnebia euchroma, Calli, Cryoprotectant, Cryopreservation

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