J Integr Plant Biol. ›› 1992, Vol. 34 ›› Issue (12): -.

• Research Articles •    

High Protein Somaclones Seleted from Somaclonal Varients in Triticum aestivum L.

Zhu Zhi-qing (C. C. Ghu), Sang Jian-li, Wang Yu-xiu, Li Yin-xin, Zhu Ying-min, Wang Pei, Fang Ren, Fan Guang-nian, Chen Yu-ron and Pei Cui-juan   

Abstract: Calli were induced from the explants of young inflorescences of a haploid wheat pollen plant of wheat (Triticum aestivum L.). The calli were subcultured and 138 regenerated plants were differentiated from them. Among the plants 5 set 42 seeds from which 34 R2 plants were obtained. The R2 plants varied in plant height, awnedness, waxiness and maturation period. In R3 generation some somaclone lines segregated but most of them were more or less stable. All clone lines were stable in R4 generation. According to the assay of seed prorein content of 82 selected clones from over 100 R5 stable clones, 5 clones with high protein content, which varied from 19.16% to 21.33%, were discovered. Repeated assay in R6 generation confirmed the results. Differences in electrophoresis pattern of seed protein and amino acid content among clones were also recorded.

Key words: Somaclonal variation, Seed protein, Wheat

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