J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (8): -.

• Research Articles •    

Localization of Heat Shock Protein of Low Molecular Weight by Electromicroscopic Immunogold-Labelling

Liu Jian, Yang Xiao-he and Wu Xian-rong   

Abstract: Inspire of the large amount of low molecular weight heat shock protein (LMW HSP) present in plant, its function has still not been clearly known. Understanding the distribution and movement of LMW HSP in cells could provide useful information about its biological functions. A 14 kD HSP was purified from the microsome isolated from the bean of a highly thermotolerant plant Phaseolus vulgaris. Antiserum against this protein was preparaed. The localization of the protein in the cell was analysed by means of electromicroscopic immunogold-labelling method. The images of electromicrograph showed that 14 kD HSP mainly existed in both cytoplasm and endoplasmic reticulum and that no labeled gold was found in tonoplasma, mitochondria or cell wall.

Key words: Low molecular weight heat shock protein, Electromicroscope immuno-gold-labelling, Phaseolus vulgaris

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q