J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (7): -.

• Research Articles •    

Effects of Mannitol Pretreatment on Androgenesis of Barley (Hordeum vulgare L.)

Li Wen-ze, Song Zi-hong, Jing Jian-kang and Hu Han   

Abstract: The effects of mannitol pretreatment on androgenesis of barley were systematically studied in comparison with that of cold pretreatment and control. The results showed that mannitol pretreatment could significantly increase the frequency of pollen survival reaching 19.0% on the eighth day, while in cold pretreatment and control they were 8.4% and 6.6 %, respectively. Mannitol pretreatment could also improve the quality of pollen and inhibit starch production from microspore, which were quite advantageous to microspore division and development. The developing period was shortened 2--3 days as compared with cold pretreatment and control. The major developmental pathways of androgenesis after mannitol pretreatment were the equal division (B pathway). In addition, the majority of microspore nuclei were diploids. On the contrary, the major microspores pretreated with low temperature had fewer chromosomes than with mannitol pretreatment, the microspore nuclei were haploids.

Key words: Barley, Anther/pollen culture, Mannitol pretreatment, Androgenesis

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