J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (8): -.

• Research Articles •    

Male Sterile Tobacco Plants Obtained by Genetical-Engineering

Li Sheng-guo, Liu Yu-le, Zhu Feng, Luo Yu-ying, Kang Liang-yi and Tian Bo   

Abstract: Chimaeric TA29-barnase gene was obtained by fusing tobacco anther-specific promoter TA29, barnase gene and NOS terminator and was inserted into a plant expression yector containing bromoxynil-resistant bxn gene and then introduced into tobacco plants. Some transgenic tobacco plants were resistant to 1.0 × 10-3 mol/L bromoxynil. At about 26℃, all of the 9 transgenic plants were male fertile; However, at 20℃, 7 out of 11 transgenic plants were male sterile.

Key words: Male sterility of tobacco plants, TA29 promoter, Barnase gene

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