J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (10): -.

• Research Articles •    

Construction of Rice cDNA Library and Partial Sequence Analysis of 100 Randomly Selected cDNA

Cao Kai-ming, Zhan Shu-xuan, Huang Wei-da, Jiang Ying, Xin Hua and Sun Chong-rong   

Abstract: A cDNA library of rice (Oryza sativa ssp. indica cv. "Guangluai 4") etiolated shoot was constructed using Lambda ZAP Ⅱ vector. After analysing the partial sequences of 100 randomly selected clones and database comparison to rice and other plants, 13 % cDNA clones could be identified and 12 % cDNAs had high degree of sequence similarity to partial sequence from rice or other species, whose function is still unknown. The remaining 75% cDNAs showed little or no similarity to genes in the database and might represent novel genes. It demonstrates the suitability of this library for large-scale sequencing from which more information of functional genes will result.

Key words: Rice: cDNA library, Sequence analysis, Comparison of homology

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