J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (9): -.
• Research Articles •
Wang Jun-hui, Yan Qing-feng and Huang Chun-nong
Abstract: Vitrification is a new technique of cryopreservation. Immature inflorescences of barley (Hordeum Vulgate L. ) were precultured in medium supplemented with 0. 5 mol/L sucrose for 2 days and dehydrated in PVS2 at 0 ℃ for 5 min before plunged into liquid nitrogen. After rapid warming and unloading, the survival of three cultivars "81Gl", "Gebeina", and "Xu 9" was 100%, 82.5% and 50% respectively. Callus formation and plant regeneration was achieved from recovered inflorescences. The non-precultured samples could not survival in liquld nitrogen. Desiccation-tolerance and freeze-tolerance affected plant survival. Vitrification shared the advantages of high levels of survival, short period of lag stage and complete viability of cryopreserved organs.
Key words: Hordeum vulgate: Immature inflorescences, Vitrification, Cryopreservation
Wang Jun-hui, Yan Qing-feng and Huang Chun-nong. Plant Regeneration from Barly (Hordeum vulgare) Immature Inflorescences Cryopeserved by Vitrification[J]. J Integr Plant Biol., 1996, 38(9): -.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.jipb.net/EN/
https://www.jipb.net/EN/Y1996/V38/I9/