J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (7): -.

• Research Articles •    

Study of a Hydridoma Cell Line Producing Monoclonal Antibodies Against Spinach Betaine Aldehyde Dehydrogenase and Its Application

Liang Zheng, Tan Ping-ping, Luo Ai-ling, Song Hai-yan, Zhang Ting and Bai Qin-hua    

Abstract: By fusion of mouse myeloma cells (SP2/O-Ag14) and spleen cells derived from BALB/c mice immunized with spinach betaine aldehyde dehydrogenase (BADH) protein, a hybridoma cell line secreting monoclonal antibodies was obtained. The antibody titer of the ascites was about 1 : 103. Not only could the monoclonal antibodes cross react with the BADH of spinach and sugar beet, it could also cross react with the leaf and root crude extracts of barley, rice, sorghum, and wheat. These results indicated the occurrence of BADH in both the photosynthetic tissue and the non-photosynthetic tissue of these graminea spicies.

Key words: Betaine aldehde dehydrogenase, Monoclonal antibody, ELISA, Gramineae crop

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