J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (6): -.

• Research Articles •    

Characterization of the Membrane- Bound Gibberellin Binding Proteins from Young Shoots of Rice

Song Ping, Cao Xian-zu, Wu Yong-hong, Zhu Xiao-hong and Liang Jian-sheng   

Abstract: Gibberellin-binding proteins were found on the membrane of young rice shoot. The dissociation constant (Kd) for GAs was approximately 6.5 × 10-8 mol/L, and the total concentration of the sites was 0. 3 pmol ·mg-1 protein. The binding activity of gibberellin-binding proteins was significantly affected by temperature and phi which was 140% higher at 0 ℃ than that at 25 ℃, and the optimal pH value was 5. Gibberellin-binding activity increased with the incubation time, reaching the maximum at 1 h. and then decreased gradually. Both IAA and ABA were able to compete with GA3 for gibberellin-binding proteins.

Key words: Rice: Gibberellin-binding protein, Binding characterization

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