J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (6): -.

• Research Articles •    

Production of Trichosanthin from the Hairy Roots of Trichosanthes kirilowii Maxim.

Qiu De-you, Zhu Cheng and Zhu Zhi-qing (Chu Chih-ching)   

Abstract: Hairy roots of Trichosanthes kirilowii Maxim. were successfully induced by infection with Agrobacterium rhizogenes strain R1601 and an in vitro culture system of thehairy roots was established. The presence of trichosanthin in the hairy roots of T. kirilowii was confirmed by western blotting. The yield of trichosanthin was determined to be 8. 16 mg/g fr. wt with SDS-PAGE gel electrophoresis and thin layer chromatography.

Key words: Trichosanthes kirilowii, Hairy roots, Trichosanthin

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