Abstract: The influences of IBA and 6-BA on the formation of grafting were studied by using explanted internode autografting of cucumber (Cucumis sativus) cultured in vitro, which was a simpler procedure for grafting with lower chance of contamination than the ordinary explanted internode grafting. Colour changes, root initiation and callus formation of the explanted internode graf related to the concentration of plant hormones added to the medium. Plant hormones controlled the formation of graft unions through influencing the time and the number of vascular bridges formation between the stock and the scion. In authors experiments, the optimal condition of plant hormone control was achieved when IBA 1.2 mg/L was added in the scion medium and 6-BA 0.3 mg/L was added to scion and stock medium.

Key words: Plant hormones, Cucumber, Explant internode grafting