J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (2): -.

• Research Articles •    

Protoplast Culture and Plant Regeneration of Ramie

Chen Xi-wen, Chen De-fu, Zhou Pu-hua and Li Zong-dao   

Abstract: Calli were induced and suspension cell lines were established from cotyledones of ramie (Boehmeria nivea). Protoplasts (2 × 10 6/g fr. wt) were isolated from suspension cell cultures in enzyme mixture solution containing 4. 5 % cellulase Onozuka R-10 and 0. 8 % Macerozyme R-10, 0.8 % hemicellulase. When cultivated on KM8p medium containing 2, 4-D 0.5 mg/L, KT 0.5 mg/L with alginate embedding method, they grew vigorously and produced microcalli within fifty days. After subcultured, the protoplast-derived ~alli produced shoots and roots on different differentiation media, then complete plants were formed. Protoplasts from cotyledones divided only several times.

Key words: Ramie, Protoplast culture, Plant regeneration

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