J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (7): -.

• Research Articles •    

Isolation and Characterization of a Lipid Transfer Protein Gene from Rice

LIU Xiao-Fei, XU Jiu-Zhen, HOU Yu-Qing, ZHAN Shu-Xuan, GE Xiao-Chun and CAO Kai-Ming   

Abstract: A lipid transfer protein gene, LTPll0, was isolated from rice (Oryza sativa L. ssp. ind/ia) genomic DNA library using a LTP cDNA(pFDRSCll0) as probe. Sequence analysis indicated that it was 1.8 kb in length and encoded a peptide of 121 amino acids. The coding region was interrupted by an intron of 90 bp. There were two canonical TATA boxes, several inverted and repeated elements and palindromic sequences at the 5' upstream and a polyadenylation signal at the 3' downstream. A signal peptide of 28 amino acids was found at its N-terminus. Homology comparison revealed that it possessed the typical features of plant LTPs.

Key words: Rice LTP gene, Regulatory elements, Protein homology

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