J Integr Plant Biol. ›› 2012, Vol. 54 ›› Issue (1): 15-32.DOI: 10.1111/j.1744-7909.2011.01084.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Characterization of the Tomato Prosystemin Promoter: Organ-Specific Expression, Hormone Specificity and Methyl Jasmonate Responsiveness by Deletion Analysis in Transgenic Tobacco Plants

Hamlet Avilés-Arnaut and John Paul Délano-Frier*   

  1. Center of Research and Advanced Studies (Cinvestav) at Irapuato: Unit for Plant Biotechnology and Genetic Engineering, Km 9.6 del Libramiento Norte Carretera Irapuato-León. Postal Box 629, Postal Code 36821, Irapuato, Guanajuato, Mexico
  • Received:2011-09-13 Accepted:2011-10-21 Published:2011-11-02
  • About author:*Corresponding author. Tel: +52 (462) 623 9636 or 623 9600; Fax: +52 (462) 624 5996 or 623 9611; E-mail: jdelano@ira.cinvestav.mx

Abstract:

Tomato systemin is a bioactive peptide that regulates the systemic activation of wound-responsive genes. It is released from its 200 amino acid precursor called prosystemin. Initial tissue-localization and hormone-induced expression assays indicated that the tomato prosystemin gene (SlPS) accumulates mainly in floral tissues and in response to exogenous abscisic acid and methyl jasmonate (MeJA) treatments, respectively. Later, the promoter regions of the PS gene in tomato (Solanum lycopersicum L. cv. Castlemart), pepper (Capsicum annuum) and potato (Solanum tuberosum) were isolated and an in silico analysis of the SlPS promoter revealed an over-representation of stress- and MeJA-responsive motifs. A subsequent 5′ deletion analysis of the SlPS promoter fused to the β-glucuronidase reporter (GUS) gene showed that the –221 to +40 bp proximal SlPS promoter region was sufficient to direct the stigma, vascular bundle-specific and MeJA-responsive expression of GUS in transgenic tobacco plants. Important vascular-tissue-specific, light- and MeJA-responsive cis-elements were also present in this region. These findings provide relevant information regarding the transcriptional regulation mechanisms of the SlPS promoter operating in transgenic tobacco plants. They also suggest that its tissue-specificity and inducible nature could have wide applicability in plant biotechnology.

Avil´es-Arnaut H, D´elano-Frier JP (2012) Characterization of the tomato prosystemin promoter: organ-specific expression, hormone specificity and methyl jasmonate responsiveness by deletion analysis in transgenic tobacco plants. J. Integr. Plant Biol. 54(1), 15–32.

Key words: cis-regulatory elements, flower specific, jasmonic acid, methyl jasmonate, promoter analysis, prosystemin, wound-responsive genes

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