J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (7): -.

• Research Articles •    

Inheritance if Chloroplast and Mitochondrial DNA in Chinese Fir(Cunninghamia lanceolata)

Qi Wen-Qing, YANG Hui-Jun, XUE Yong-Biao and HU Shi-Yi   

Abstract: The inheritance of mitochondrial (mt) DNA and chloroplast (cp) DNA was investigated in intergeneric hybrids from crossing between Cunninghamia lanceolata (Lamb.) Hook. and Cryptomeria fortuaei Hooibrenk. The chloroplast tmL-trnF region and one intra-genic segment of the mitochondrial gene, Cox m, were amplified from those of the parents and hybrids by PCR using gene-specific primers. Cp- and mtDNA polymorphisms of the amplified regions were detected between the parents after restriction digestions. Restriction fragmem length polymorphism (RFLP) analysis revealed that all the Fl individuals possessed Cox Ⅲ restriction fragment patterns (characteristic of the paternal parent Cryptomeria fortunei ) and the tmL-tmF region (identical to the matemal parent Cunninghamia lanceolata ) showing that a different mode of inheritance for organelle DNA has occurred in the hybrids. Furthermore, the maternal inheritance of chloroplast DNA is reported here for the first time in coniferophyta.

Key words: Curminghamia lanceolata, Cryptomeria fortunei, Inheritance of chloroplast DNA, Inheritance of mitochondrial DNA, Restriction fragment length polymorphism

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q