Abstract: The chloroplasts of wheat have chanced greatly at high temperature condition(34℃). When wheat grown at 34℃ for 10 days, its chlorophyll content was 6 times less than that under the normal condition(22℃). The ribosomes were isolated from the leaves by sucrose density gradient centrifugation. It is found that only 80 S ribosomes existed in wheat leaves grown at the high temperature and the formation of 70 S ribosomes is specifically prevented. Since the absence of 70 S ribosomes in chloroplast, proteins synthesis can no longer proceed. Analysis of SDS-polyacrylamide gel electrophoresis indicates that the bands of chloroplast proteins from the leaves of wheat at the high temperature are less than those under normal condition. One of the poly- peptides the large subunit(MW=57000 daltons) of ribulose bisphosphate carboxylase, which is coded for by chloroplast genome and synthesized on 70 S ribosome in chloroplast, was lost. The photosynthetic intensity is decreased due to the blocking synthesis in chloroplast of some polypeptides which play the important role in photosynthesis.

Key words: Wheat, Hight temperature, Chloroplast ribosomes, Chloroplast protein, Biosynthesis