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J Integr Plant Biol, 1984, 26 (4): -, Research Article
Changes of Ribonuclease in Cotyledon Segment of Phaseolus radiatus L During Dedifferentiation
Zhang Jing-lan, Xu Gui-fang, Tang Ding-tai and Niu Yu-xian
doi:
Abstract
Ribonuelease activity and its isozyme pattern of mung bean cotyledon segment were determined during dedifferentiation. The results showed that the activity of ribonuelease in the dedifferentiated tissue is higher than that of control, three new bands of isozyme appear during callus formation. Addition of cycloheximide (CHM) at 0 or 24 hours inhibits either the enzyme activity or the appearance of new bands. These facts indicate that the changes of ribanuelease are at translation level.
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