J Integr Plant Biol. ›› 1984, Vol. 26 ›› Issue (2): -.

• Research Articles •    

Effects of Physical and Chemical Factors on Induction Frequency of the Pollen Plantlet and Changes in Starch Formation in Anthers

Gu Shu-rong, Gui Yao-lin and Xu Ting-yu   

Abstract: Physical and chemical facttors affected distinctly induction frequency of the pollen plantlets from anther culture in vitro of Lycium. Experimental results showed that the anthers with their pollen grains at the uninucleate stage when the nuclei were centrally situated, were the best material for the anther culture. The different proportions of various hormones have affected the embryoid formation. When the MS medium was supplemented with 6-BAP (1 ppm) and NAA (0.1 ppm), the induction frequency was increased ( 16.9% ). When 3–15 per cent of sucrose was added in medium, the embryoids were induced and 15 per cent was the optimum. The callus of the filament was inhibited by the increase of the sucrose concentration. Before inoculation the anthers were pretreated at 3–5℃ for 4 days, the frequency of embryoid formation was efficiently increased in comparision with those of untreated anthers. The induction frequency of normal anthers was only 2.8 per cent, but that of the anthers pretreated was 3–9 per cent, the highest was 8.9 per cent .The changes of substances in anther pretreated and in normal anther was compared by means of histochemistry. Under normal conditions, there were a lot of starch accumulated in the inner wall Of the anthers and the distribution of the cytoplasm and the staining of the protein were even: In the anther pretreated, the starch grains have disappeared and the cytoplasm has condensed and the staining of the protein wasn't even. The differences may be related to induction, frequency of the anther culture.

Key words: Lycium, Anther Culture, Starch

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