J Integr Plant Biol. ›› 1983, Vol. 25 ›› Issue (6): -.

• Research Articles •    

Cryopreservation of Calli and Their Suspension Culture Cells of Anisodus acutangulus

Zheng Guang-zhi, He Jing-bo and Wang Shi-ling   

Abstract: Calli and their suspension culture cells of Anisodus acutangulus cultures which had been cryopreservated showed no change in cell morphology or growth potential in culture and retained biosynthesis ability of hyoscyamine and scopolamine. Cells viability beyond 90% has been obtained after cryopreservation. In cryoperservation experiments, calli were better than their suspension culture cells, the younger was better than the older, –196 ℃ was better than –20 ℃, cryoprotectant lactalbumin hydrolysate was similar to the dimethyl sulfoxide. Calli and their suspension culture cells of Al acutangulus could not be cryopreservated in the presence of cryoprotectant glycerine or in the absence of cryprotectant.

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