J Integr Plant Biol. ›› 1983, Vol. 25 ›› Issue (6): -.

• Research Articles •    

Histochemical Studies of Scutellum During Germination of Oat Seed by Light Microscopy and Immunofluorescent Localization of Oat Globulin

Xu Shi-xiong(S. Y. Zee), Chen Qing-rang( H. Y. Chan) and Ma Zheng-yong(C. Y. Ma)   

Abstract: The protein, phytin, lipid and starch contents of the scutellum of oats showed marked changes during the first three days after seed germination. Protein and phytin disappeared almost completely during the first two days after seed germination. The degradation at lipid was much slower. In the scutellum of the ungerminated seed very few starch granules were seen. At days-1 and -2 after germination the starch contents increased, but at day-3 the starch contents decreased. Immunofluorescent localization of oat globulin indicated that the oat globulin was sequestered mostly, if not exclusively, in the protein bodies. The degradation of the oat globulin inside the protein bodies was very rapid. At day-3 after seed germination it disappeared almost completely. When excited by the ultraviolet light the walls of both the epithelial and parenchyma cells of the scutellum autofluoresced intensely. As germination progressed further, the autofluorescence in the walls of the epithelial cells gradually faded away, whereas the autofluorescence in the walls of the parenchyma cells did not.

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