J Integr Plant Biol. ›› 1965, Vol. 13 ›› Issue (2): -.

• Research Articles •    

The Effect of Osmotic Value of the Medium on the Growth of the Young Sunflower Embryos Cultured in Vitro

J. S. Kuo and F. H. Wang   

Abstract: The present paper deals with the effect of osmotic value of the medium on the growth of the young sunflower embryos grown in vitro. Based upon the results of a series of experiments carried out during 1963 the following conclusions are reached: 1. The sunflower embryos grown in vitro require different concentrations of sucrose, varying with the age at which the embryos are excised. Generally speaking, the optimum concentration of sucrose for the embryos 1,000–1,100μ in length is about 17.5%, for 2,000–2,500 μ, about 16% and for 5,000–5,500 μ, about 12.5%. 2. The sucrose may be partially replaced by sodium chloride (0.2%–0.4%) or mannitol (1.125%–5.5%) as an osmotically active substance, but 0.8% sodium chloride is apparently toxic to the young embryos. 3. Embryos cultured in hypotonic medium usually germinate precociously, those in approximately isotonic medium continue their embryonic growth, while those in hypertonic medium become more or less checked in their growth.

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