J Integr Plant Biol. ›› 1983, Vol. 25 ›› Issue (3): -.

• Research Articles •    

Studies on Fatty Acids and Unsaponifiable Constituents of Oil from Tubers of Cyperus esculentus L.

Yan Mci-he and Guan Zheng-xue   

Abstract: The fatty acids of the oil from tubers of Cyperus esculentus L. were determined by gas chromatography with DC-11 and DEGS stationary phases. Oleic, linoleic, palmitic and stearic acids are the major constituents in the fatty acid fraction, while lauric, myristic, linolenic, arachidic, dadoleic, behenic and tetracosanoic acids are the minor ones. The unsaponifiable matters of the oil were separated by column chromatography with silica gel and thin layer chromatography with silica gel G into three fractions: sterols, 4-methylsterols and triterpene alcohols. The acetates of sterols, 4-methylsterols and triterpene alcohols were separated by TLC with 20% silver nitrate impregnated silica gel G, using CH2Cl2-petroleum system as developing reagents. The identification of major components was carried out by TLC, mp, optical rotation, GLC, IR spectrum and GC-MS. It was found that β-sitosterol, stigmasterol and campesterol were present in large amounts in the unsaponifiable fraction, β-sitosterol, stigmasterol, △5-and △7-avenasterol, 24- methylenecholesterol and 24-methylenecholest-7-enol in the sterol fraction, obtusifoliol, gramisterol and citrostadienol in the 4-methylsterol fraction, and cycloartanol, cydoartenol, 24- methylenecydoartanol and cyclobranol in the triterpene alcohol fraction were isolated and identified, while campesterol, campestanol, stigmastanol, △7-stigmastenol, △7-campestenol and △7-cholestenol were identified only. We found no evidence of the occurence of nonedibles in this oil.

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