Abstract: 1. There are mainly two modes of first divisions from pollen cells diverted into sporophytes in anther culture of wheat, i.e. equal and unequal divisions. According to Feulgen reaction of its daughter nuclei and whether they participate in the formation of multicellular pollen or not, we distinguished four basic types of the abnormal pollen, i.e. type A, B. C and D. C and D are the types in which their generative nuclei were involved in the formation of multicellular pollen. 2. Before inoculation, the excised anthers on Ne liquid medium supplemented with 10% sucrose were subjected to a pretreatment for 72 h at 3–5 ℃, then the anthers were suspended on N6 liquid medium containing 12 mg/L IAA, 2 mg/L kinetin, 300 mg/L casein hydrolysate and 10% sucrose. Under these conditions the mean amount of multicellular pollen grains per anther might be increased to 21.42. 3. We also found that the early development of pollens was related to the viability of anther wall tissue. Comparatively, higher exogenous hormones could keep viability and prolong the life of anther wall cells.