J Integr Plant Biol. ›› 1982, Vol. 24 ›› Issue (6): -.

• Research Articles •    

Changes of Adenylate Pool and Energy Charge E. C. in Relation to the Germination of Chinese Cabbage Seeds

Shen Quan-guang, Liu Cun-de, Zhang Jia-yuan, Yan Tian, Yan Long-fei and Tang Pei-song   

Abstract: The changes of adenylate pool (ATP, ADP, AMP) and energy charge (E. C.) were determined in germinating seeds of Chinese cabbage. The dry seeds contained a few ATP, most of the adenylate was in the form of AMP. After 1 h of inhibition, most of AMP was converted to ATP, and E. C. value increased rapidly. If the inhibition continued for two hours, the level of ATP increased about 35-fold, and E. C. value reached 0.51. After 24 h of germination, the ATP level in the non- germinating seeds was lower 4 times than that in the germinating seeds and E. C. value was lower too. If the seeds germinated in 3% O2, the ATP level dropped. As the seeds were transfered to air, the adenylate pool and E. C. value increased to the level of the control, then the percentage germination reached 76%. When the seeds were treated with 3% O2, 5 × 10-4 M 2, 4-dinitro phenol, 1 × 10-5 M earbonyl cyanid m-chlorophenyl hydrozone and 5 × 10-3 M iodoaeetie acid at the first 1–2 h, they inhibited the production of ATP and decreased E. C. value. Iodoaeetic acid was the most effective one among the four inhibitors. It was shown that the rate of germination was closely related to the energy charge and the adenylate pool.

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