Please wait a minute...
J Integr Plant Biol, 1982, 24 (4): -, Research Article
Preliminary Studies on the Reactivation of MoFe Protein Denatured by O2 with FeMoCo
Huang Ju-fu, Luo Ai-ling, Zhang Hui-miao, Zhou Jing-dong, Li Yong-xing and Liang Yin-chu
doi:
Abstract
After exposure to air, the crystalline MoFe protein from Azotobacter vinelandii did not degenerate to any fractions containing Mo, Fe atoms. FeMoCo, extracted from the MoFe protein with organic solvents, was able to reeactivate the denatured MoFe protein, and the acetylene-reduction activity was restored fully or significantly, and the effciency of reactivation for FeMoCo decreased as the time of exposure to air inereased. The results showed that during exposure to air FeMoCo was the first fraction denatured, the other fractions in MoFe protein were denatured with the incasing time of exposure to air.
--------------------------------------------------------------------------------------------------------------------
   Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Huang Ju-fu
   Articles by Luo Ai-ling
   Articles by Zhang Hui-miao
   Articles by Zhou Jing-dong
   Articles by Li Yong-xing
   Articles by Liang Yin-chu
PubMed
   Articles by Huang Ju-fu
   Articles by Luo Ai-ling
   Articles by Zhang Hui-miao
   Articles by Zhou Jing-dong
   Articles by Li Yong-xing
   Articles by Liang Yin-chu
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22