J Integr Plant Biol. ›› 1982, Vol. 24 ›› Issue (4): -.

• Research Articles •    

Preliminary Studies on the Reactivation of MoFe Protein Denatured by O2 with FeMoCo

Huang Ju-fu, Luo Ai-ling, Zhang Hui-miao, Zhou Jing-dong, Li Yong-xing and Liang Yin-chu   

Abstract: After exposure to air, the crystalline MoFe protein from Azotobacter vinelandii did not degenerate to any fractions containing Mo, Fe atoms. FeMoCo, extracted from the MoFe protein with organic solvents, was able to reeactivate the denatured MoFe protein, and the acetylene-reduction activity was restored fully or significantly, and the effciency of reactivation for FeMoCo decreased as the time of exposure to air inereased. The results showed that during exposure to air FeMoCo was the first fraction denatured, the other fractions in MoFe protein were denatured with the incasing time of exposure to air.

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