J Integr Plant Biol. ›› 1982, Vol. 24 ›› Issue (2): -.

• Research Articles •    

Study on the Chromosome N-Banding in Plants

Zhang Zi-li, Dong Guang-yuan and Lu Ping   

Abstract: The present paper is dealing with the investigation of the chromosome N-banding technique and the N-banding patterns in Hordeum vulgare, Triticum aestivum, Secale cereale, Vicia faba and Allium eepa. Two N-banding techniques were applied. First, the chromosome slides were stained with Giemsa solution. Second, the slides were treated in 1 M NaH2PO4 solution at 92—94 ℃ for 3.5—8.5 min. After rinsing in tap water they were stained with Giemsa solution. The experiments have demonstrated that the N-banding technique is simple and rapid and the banding patterns are distinctive. The data of N-banding patterns indicated that the N bands did not display the nucleolus organisers exclusively. The comparison of the N-banding patterns of these plants with their C-banding patterns shows that in some of these plants although some regions of N-bands and C-bands correspond, there are a number of instances where regions show N-bands but no C-bands and vice-versa. Therefore, a combination of the N-banding and C-banding techniques should be valuable in the cytological identification of plant chromosome. Like the C-bands, the N-bands are also useful markers in cytogenetics.

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