J Integr Plant Biol. ›› 1981, Vol. 23 ›› Issue (5): -.

• Research Articles •    

A Study of Maize Pachytene Chromosome by Giemsa C-Banding Technique

Gu Ming-guang, Y. C.Ting and Zhang Xue-qin   

Abstract: The main results obtained are summarized as follows: The two maize strains employed in the present study were Wilbur's Flint and Pa-tang-pai 1. Wilbur's Flint From pachytene analysis it was previously known that Wilbur's Flint has only one terminal knob in the short arm of chromosome 9 as mentioned in "materials and methods" section. By Giemsa C-banding technique it revealed more bands (knobs) than expected. In addition to the terminal knob (band) in the short arm of chromosome 9, the following bands (knobs) were observed. The short arm of chromosome 1 possessed a small band. The same chromosome had another band in the 0.4: position of the long arm. Chromosome 2 also had a band in the long arm. This band occupied the 0.15 position of the arm. The bands in the long arm of chromosome 6 responded differently to the staining. The first band stained heavily while the second one only slightly. Chromosomes 7 and 10 had a band in the long arm. The band in the long arm of chromosome 10 was near the centromere. The while the terminal band of chromosome 9 showed more heterochromatin than that revealed by the other technique. The NOR (nucleolar organizer region) and the satellite of chromosome 6 were also differentiated. The centromere regions of all the chromosomes were not stained and completely indistinct. The nueleoli were not or only slightly differentiated for most of the cases. 2. Pa-tangpai With acetocarmine squash technique it had known that chromosomes 1 and 4 have each one large knob in the long arm. The knobs in the long arm of chromosomes 6 and 7 are medium-sized. The short arm of chromosome 9 has only a small knob. By Giemsa C-Banding technique it was surprising to know that the number and position of bands agrees fully to anticipation even though the size of them was not entirely the same as expected. For example, the bands (knobs) in the long arm of chromosomes 1, 4 and 7 are much larger than those revealed by the other staining methods. The terminal band (knob) in the short arm of chromosome 9 and the first (knob) in the long arm of chromosome 6 appeared the same as observed with conventional techniques. Furthermore, it is the same as for Wilbur's Flint, that NOR and satellite of Chromosome 6 were clearly differentiated. The centromere region of all the chromosomes could not be recognized and the nucleoli were almost completely unclear.

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