J Integr Plant Biol. ›› 1980, Vol. 22 ›› Issue (4): -.

• Research Articles •    

Studies on the Induction of Pollen Sporophyte of Coix lacryma Jobic L.

Wang Jing-ju, Chu Zhi-ging and Sun Jing-san   

Abstract: The developmental pathways of pollen sporophyte in anther culture of Coix were observed. The types of androgenesis are different, and are relative to the degree of the differentiation in pollen cells. Pollen-inductors develop via multicellular mass into embryoids or calli. Both of them can develop into plantlets, but the frequency of the regeneration of the plantlets in calli is higher than that of in embryoids, because there are a lot of aberrant embryoids in the latter, which cannot develop further. It was found that the induction frequency of the polleninductors can be increased apparently by the pretreatment in a short time with a hypertonic sucrose solution. The chromosomes of the somatic cells in l0 plantlets were examined. It was found that all the plantlets derived from the embryoids are haploids, while there are haploid, diploid and mixoploid in the plantlets from the calli. The effects of anther wall cells and the stability of haploid cells were discussed.

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