J Integr Plant Biol. ›› 1978, Vol. 20 ›› Issue (3): -.

• Research Articles •    

The Study of Screening Medium by Means of Balanced Incomplete Blocks (B. I. B)Design in Raising the Efficiency of Anther Culture of Rice

Laboratory of Genetics, South China Institute of Botany, Academia Sinica, Department of Mathematics, Normal College of South China   

Abstract: The result of balanced incomplete blocks (B. I. B.) design for screening medium of anther culture of rice (Oryza sativa L. subsp. Shien) indicated that the efficiency of anther culture of rice (Shien) was markedly raised when both the media "He5" and "XM2" were used as induced medium and regenerated medium respectively. The mean frequency of callus from the anther and green pollen plantlet from the callus reached 5.4% and 24.6% respectively. The result of analysis of variance proved that the donor plant materials had greater variance than the variance of medium as for inducing callus from anther and that the medium had greater variance than the variance of plant materials for differentiation of green plantlets. In the Shien rice the constituents of "induced medium" had a greater effect on the callus induction as well as to the green plantlet regeneration. When "He5" and "XM2" media were used as induced medium and differentiated medium respectively for the anther culture oil Shien rice showing broad adaptapility. It is concluded that both media, the "He5" and the "XM2", are comparatively suitable for anther culture of Shien rice. The test indicated that the orthogonal experiment combined with B. I. B. design is an effective method for screening medium of anther culture. The constituents of the two media are as follows: 1. "He5" an induced medium (mg/l) (NH4)2SO4 231; KNO3 3181.5, KH2PO4 600, MgSO4·7H2O 35; CaCl2·2H20 166; Na2 EDTA 74.5; FeSO4·7H20 55.7; MnSO4·4H2O 4.4; ZnSO4·7H2O 1.5; H3BO3 1.6; KI 0.8; thiamine HCl (B1) 0.6; pyridoxin HCl (B6) 0.6; nicotinic acid (pp) 3.0; glycine 2; casein hydrolysate 300; yeast extract 1360; 2,4-D2; NAA 2; kinetin 3; sucrose 60000 (6%) agar 9000; pH 5.8–6. 2. "XM2 (Xian Miao 2)" a regenerated medium (mg/l) KNO3 1900; NH4NO3 4950; KH2PO4 510; CaCl2·2H2O 440; MgSO4.7H2O 370; Na2· EDTA 74.5; FeSO4· 7H2O 55.7; MnSO4· 4H2 O 22.3; ZnSO4·7H2O 8.6; CuSO4.5HO2O 0.025; H3BO3 6.2; KI 0.83; Na2MoO4·2H2O 0.25; CoC1·6H2O 0.025; myoinositol 100; glycine 20; thiamine HC1 1.0; pyridoxin HCl 5.0; nicotinic acid 5.0; NAA 1.0; kinetin 2.0; sucrose 30000 (3%); agar 9000; pH 5.8-60.

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