J Integr Plant Biol. ›› 1978, Vol. 20 ›› Issue (3): -.

• Research Articles •    

A Preliminary Observation on Histogenesis and Organogenesis of the in vitro Development from Rice Microspores into Plantlets

Zhang Xin-ying, Liu De-rain and Wang Ying-li   

Abstract: The histogenesis and organogenesis during the process of the development of rice anther cultured in vitro have been investigated. The culture medium used was N6. And microscopic observations both by squash preparations and paraffin sections were made. The results obtained were as follows: The process of the development of the microspore to the formation of pollen plant could be divided into 2 stages, i.e., stage of callus formation and stage of differentiation of the adventitious buds and roots. At first, the uninucleate microspore divided into two equal daughter cells, then by repeated divisions of these two cells the callus was formed. The adventitious roots emerged from the callus were endogenesis in origin. However, the buds were exogenesis originated from a part of the peripheral cells of the callus, which by means of dedif- ferentiation had become meristematic in character. At the very beginning, adventitious roots and buds originated independently, without any connection of vascular tissues between them. Superficially, the emergence of buds is prior to that of roots in the course of pollen plant development, but anatomically, the origination of adventitious roots seems to be earlier than that of buds.

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