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J Integr Plant Biol, 1978, 20 (1): -, Research Article
Cytological Investigation on Androgenesis of Triticum aestivum
Zhu Zhi-qing, Sun Jing-san and Wang Jing-ju
doi:
Abstract
The conditions for inducing pollen embryoids and calluses and the cytological observations of androgenesis in wheat have been studied. Results are summarized as follows: 1. N6 mediumTM is apparently superior to MS medium for inducing pollen calluses. It is shown that the exogenous hormones are not necessary to initiate the androgenesis, but they can promote further growth of pollen embryoids and calluses. 2. The pathways of the androgenesis have been also investigated with the total- mount of the anther. The asymmetric and symmetric divisions in uninucleate micros- pores all can initiate embryogenesis, but the former is more frequent. 3. The micronuclei cleft from chromosome fragments, have been found both in multicellular pollen and in some albino pollen plants. It is deduced that the albino and abnormal pollen plants may originate from the multicellular pollen which contain micronuclei. 4. Both the endoduplication of the nucleus and fusion of free nuclei of pollen are believed to be the main causes for the production of the diploid and multiploid pollen plants.
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