J Integr Plant Biol. ›› 1973, Vol. 15 ›› Issue (1): -.

• Research Articles •    

Investigations on the Induction and Morphogenesis of Wheat (Triticum vulgare)Pollen Plants

Chu Zhih-ching, Wang Chin-chü, Sun Chin-san, Chien Nan-fan, Yin Kwang-chu and Hsü Chen   

Abstract: The development of haploid plants has been successfully induced from wheat (Triticum vulgare) pollens by means of the anther cultured in vitro. On the MS medium supplemented with 2–20 mg/l 2,4-D and various organic addenda, out of 21094 inoculated anthers taken for culture from 27 hybrids or varieties, 103 produced calli. All the calli developed from dehisced anther sacs. Microscopical examinations indicated that these calli were formed by repeated divisions of the pollengrains. 5 days after, the pollen calli were transferred into the medium containing either 0.2–2mg/l NAA and 0.2–2mg/l kinetin or 0.5 mg/l IAA and 15% cocoanut milk, differentiation of shoots began to occur. It was also found that if the anthers were inoculated on the MS medium containing either 20% cocoanut milk or 2 mg/l IAA and 2 mg/l kinetin, shoots would develop directly from the anther sacs. The chromosome counts in root tips of 6 plantlets examined gave the number 21, confirming that they are haploids. The haploid plants produced spikes but did not setseeds.

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