J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (12): -.

• Research Articles •    

A BAC Library Constructed to the Rice Cultivar

PENG Kai-Man, ZHANG Hong-Bin and ZHANG Qi-Fa   

Abstract: "Minghui 63" is the restorer line for a number of the most important commercial rice hybrids varieties in China. To facilitate long-term commitment in genetic analysis and molecular cloning of the superior genes in the genome of "Minghui 63", the authors have constructed a largeinsert genomic DNA library using the bacterial artificial chromosome (BAC) cloning vector (pBe- loBAC 11). Size fractionated Hind m digest of genomic DNA was ligated to the BAC vector, and the ligation mixture was used to transform the bacterial strain DH10B. A total of over 26 000 clones were obtained with the average insert size of about 150 kb, ranging from 90 to 240 kb. These clones thus represent 9 x rice haploid genome equivalents. The library is now being used for physical mapping of several genomic regions for map-based gene cloning.

Key words: Bacterial artificial chromosome library, Genome analysis, Gene clone

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