J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (8): -.

• Research Articles •    

Studies on the Kinetic Properties of Photosystem Ⅱ Primary Reaction Using Fluorescence Spectroscopy with 470 fs Time Resolution

HOU Jian-Min,KUANG Ting-Yun, CHEN Yao-Dong, YE Tong, YANG Kun-Yun, CUI Yan, TANG Chong-Qin, WANG Shui-Cai, HOU Xun and TANG Pei-Song   

Abstract: The primary reaction kinetics of the isolated photosystem Ⅱ particles and photosystem Ⅱ core complexes from spinach ( Spinacia deracea Mill. ) was investigated using the time-resolved fluorescence spectroscopy with 470 fs time resolution. 2 to 4 lifetime components were detected by the multi-exponential curve fining method. These components were analyzed and discussed in terms of different kinetic processes. It is suggested that 3 ps component is attributed to the charge separation and 0.8 ps, 12 ps, 25 ps and 100 ps components are related to the energy transfer processes. A possible kinetic scheme in photosystem Ⅱ reaction center was proposed based upon the reported previously result.

Key words: Photosynthesis, Primary reaction, Photosystem Ⅱ, Time-resolved spectroscopy, Kinetics

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