J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (8): -.

• Research Articles •    

Culture of Transgenic Artemisia annua Hairy Root with Cotton Cadinene Synthase Gen

CHEN Da-Hua, MENG Yu-Ling, YE He-Chun, LI Guo-Feng and CHEN Xiao-Ya   

Abstract: A cotton cadinene synthase gene was transferred into Artemisia annua L. via Agrobacterium rhizogenes. The cotton cDNA cad-C14 was placed in binary vector under the 35S promoter. Leaf explants were infected with Agrobacterium rhizogenes 15834 containing binary vector to induce the hairy roots. 121 hairy root lines with rapid growth were established. A concentration of 20 mg/ L kanamycin was added in order to select the transformed hairy roots. 12 hairy root lines were selected, which were resistant to kanamycin. Polymerase chain reactions and Southern blotting revealed that 3 % of the hairy roots (four hairy root lines ) contained the foreign gene. The results of RT-PCR suggested that the foreign CAD gene of the transgenic hairy root had expressed at the transcriptional level. An efficient Agrobacterium rhizogenes-mediated transformation system of Artem/s/a annua was established.

Key words: Artemisia annua, Cadinene synthase, Transgenic hairy root

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22