J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (1): 93-97.

• Research Articles • Previous Articles     Next Articles

Cloning and Differential Expression of Two Catalases in Suaeda salsa in Response to Salt Stress

MA Chang-Le,WANG Ping-Ping,CAO Zi-Yi,ZHAO Yan-Xiu,ZHANG Hui   


Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2_scavenging enzyme, were isolated from a λZap_cDNA library constructed from a 400 mmol/L NaCl_treated library of Suaeda salsa (L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady_level of both genes in roots after 48 h salt treatment, but only Sscat1 was induced in salinity treated leaves. Time_course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.


作者:马长乐 王萍萍 曹子谊 赵彦修 张慧


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