J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (4): 399-402.
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WANG Qi-Hua, ZHANG En-Dong, LI Da-Peng, CHENG Ai-Hua
Abstract:
Dunaliella salina (Dunal) Teod. and Platymonas helgolandica Kylin var. tsingtaoensis were cryopreserved in liquid nitrogen with a two-step cryopreservation method. With 5% and 20% dimethylsulphoxyde (DMSO) concentration respectively through 30 min equilibrium time, and under -40℃, 60 min and -30℃, 30 min respectively as prefreezing temperature and sustained time, and by using slow dilution method to remove DMSO from the sample at 0℃ and room temperature respectively after sample thawing, D. salina and P. helgolandica var. tsingtaoensis gained the highest survival rates that were 68.5% and 78.3% respectively.
盐生杜氏藻和青岛大扁藻的超低温保存 王起华 张恩栋 李大鹏 程爱华 (辽宁师范大学生物系,大连116029)
摘要:采用两步法冷冻技术超低温保存盐生杜氏藻 (Dunaliellasalina (Dunal)Teod .)和青岛大扁藻 (Platymonashel golandica Kylinvar.tsingtaoensis)。当二甲基亚砜 (DMSO)浓度分别为 5 %和 2 0 % ,平衡时间均为 30min ;预冻温度及保持时间分别为 - 40℃ ,6 0min和 - 30℃ ,30min ;化冻后分别在 0℃和室温下采用慢速稀释法去除DMSO时 ,盐生杜氏藻和青岛大扁藻都达到了最高存活率 ,分别为 6 8 5 %和 78 3%。
关键词: 盐生杜氏藻;青岛大扁藻;超低温保存(液氮)
Key words: Dunaliella salina , Platymonas helgolandica, cryopreservation (liquid nitrogen)
WANG Qi-Hua, ZHANG En-Dong, LI Da-Peng, CHENG Ai-Hua. Cryopreservation of Dunaliella salina and Platymonas helgolandica var. tsingtaoensis[J]. J Integr Plant Biol., 2000, 42(4): 399-402.
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