Abstract:

An approximately 800 bp cDNA (Lhcb 2) encoding light-harvesting chlorophyll a/b-binding protein complex (type Ⅱ) was cloned from the seedling of pea (Pisum sativum L.) with RT-PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb 2 in pea genome. RT-PCR and Northern blotting revealed the expression of Lhcb 2 which was regulated by light in a time-dependent expression manner. The Lhcb 2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4℃) also affected the Lhcb2 gene by decreasing half of its expression under 25 ℃.

光照和温度对豌豆Lhcb2基因表达的影响
孙钦秒 李良璧*  阎久 胜毛大璋 匡廷云
（中国科学院植物研究所光合作用研究中心，北京100093）

摘要：采用RT-PCR技术 ,从豌豆 (Pisumsativum L .)幼叶中克隆了 1个约 80 0bp的Lhcb2cDNA。以特异探针进行的Southern杂交结果表明 ,Lhcb2基因以单拷贝形式存在于豌豆基因组中。不同光照时间和温度对豌豆幼苗进行处理的RT_PCR和Northernblotting分析表明 ,Lhcb2基因转录水平上的表达受光照的控制 ,且明显地表现出对光照时间的依赖性。光照 0～ 1.5hLhcb2基因未见表达 ,而光照 2h以上该基因才大量表达 ;温度对Lhcb2基因的表达亦有显著的影响 ,相同的光照处理 ,4℃下Lhcb2基因的表达量比 2 5℃下的表达量低 1倍左右。
 

关键词： 豌豆；Lhcb2 基因；光照和温度；转录表达

Key words: pea, Lhcb 2 gene, light and temperature, expression