J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (9): -.

• Research Articles •    

An Intron Sequence is Present in the 3 UTR of Tomato Cf9 Gene

YANG Chong-Lin, WANG Min, LI Yi, QU Li-Jia, GU Hong-Ya and CHEN Zhang-Liang   

Abstract: Tomato leaf mould-resistance gene Cf) was amplified by PCR and cloned from the genomic DNA of a tomato ( Lycopersicon esculentum Mill. ) cultivar ("Zhongza 9'). Sequence analysis indicates that this Cf9 gene is 2 751 bp long and contains an open reading frame encoding a protein of 863 amino acids. An intron of 115 bp in length is found in the 3' untranslated region of this gene. The exon/intron borders are replicate sequences, TCCAGG(T)ATTC, which shares high homology with those of the two genes of Cf2 which is another leaf mould-resistant gene locus in tomato. Compared with the previously reported cDNA sequence of Cf9, a single change of T to C occurs at the nucleotide position of 371 in this PCR-ampliiied Cf) gene, which converts Leu 121 to Pro in the LRR region of the encoded protein.

Key words: PCR, Tomato, Resistance gene, Intron

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