J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (9): -.

• Research Articles •    

Rice Transformation with a Phytoalexin Gene and Bioassay of the Transgenic Plants

TIAN Wen-Zhong, DING Li, CAO Shou-Yun, DAI Shun-Hong, YE Song-Qing and LI Liang-Cai   

Abstract: Immature embryos, mature embryos and embryogenie ealli of 6 rice ( Oryza sativa L. ) materials were transformed with particle bombardment. The plasmids pSSVsfl and pVE5 + were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 ( 100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae) and bacterial blight of rice ( Xanthomonas oryzae). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.

Key words: Rice transformation, Phytoalexin, Stilbene synthase, Resistance to blast and bacterial blight of rice

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