J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (9): -.

• Research Articles •    

Study on Regulation of 5′ Flanking Regions of Granule-bound Starch Synthase Gene of Potato

SONG Dong-Guang, SUN Guo-Feng, SHAN Hai-Yan, PENG Xiang-Yin, WANG Guang-Qing, WANG Xun-Ming and TAN Jia-Zhen   

Abstract: Binary vectors were constructed by fusing 0.4, 0.8, 1.6, and 2.9 kb 5' flanking regions of GBSS gene with GUS (β-glucuronidase). Transient GUS expression was observed in in vitro tuber slices bombarded with 0.8 kb GBSS-GUS construct. These constructs were then transferred into potato ( Solanum tuberosum L. cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confmned by X-Gluc staining and PCR. Using in vitro tuberization system, GUS expressions were assayed with fluorescence, it was shown that 0.8, 1.6 and 2.9 kb GBSS-GUS expressions were higher than 0.4 kb GBSS-GUS. 1.6 and 2.9 kb GBSS-GUS expressions were about 2 to 10-folds higher in tubers than in stems. In cultured shoots, GBSS-GUS expression could be induced by increased sucrose concentration but inhibited by light.

Key words: Gene regulation, Granule-bound starch synthase, Tuber-specific expression, in vitro tuberization, Solanum tuberosum

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