J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (3): 289-297.DOI: 10.1111/j.1744-7909.2010.00890.x

• Plant-environmental Interactions • Previous Articles     Next Articles

Expression of Acyl-lipid Δ12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato

Reza Maali Amiri1*, Natalia O. Yur'eva2, Khristina R. Shimshilashvili3, Irina V. Goldenkova-Pavlova3, Vasiliy P. Pchelkin2, Elmira I. Kuznitsova2,Vladimir D. Tsydendambaev2, Tamara I. Trunova2, Dmitry A. Los2, Gholamreza Salehi Jouzani4 and Alexander M. Nosov5   

  1. 1Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources of the University of Tehran, Karaj 31587-77871, Iran
    2K.A.Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (RAS), Moscow 127276, Russia
    3Vavilov Institute of General Genetics, RAS, Moscow 119991, Russia
    4Agricultural Biotechnology Researches Institute of Iran (ABRII), Karaj 21525-1867, Iran
    5Department of Plant Physiology, Moscow State University, Moscow 119992, Russia
  • Received:2009-01-04 Accepted:2009-09-21 Published:2010-03-01
  • About author: *Author for correspondence Tel: Tel: +98 261 224 6074; Fax: +98 261 222 7605; E-mail: rmamiri@ut.ac.ir

Abstract:

We report the expression profile of acyl-lipid Δ12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cellmembrane lipid composition and cold tolerance in prokaryotic (Escherichia coli) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants was observed for lines 2 (73%) and 3 (41%). Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction.
 

Maali Amiri R, Yur’eva NO, Shimshilashvili KR, Goldenkova-Pavlova IV, Pchelkin VP, Kuznitsova EI, Tsydendambaev VD, Trunova TI, Los DA, Salehi Jouzani G, Nosov AM (2010) Expression of acyl-lipid Δ12-desaturase gene in prokaryotic and eukaryotic cells and its effect on cold stress tolerance of potato. J. Integr. Plant Biol. 52(3), 289–297.

Key words: Acyl-lipid Δ12-desaturase, E. coli, fatty acids, Solanum tuberosum;thermostable lichenase, transformation.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22