J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (4): -.

• Research Articles •    

Expression of Mouse MT-cDNA in Filamentous Cyanobacterium to Enhance Its Metal- resistance

GUO Xiang-Xue, ZHAO Hui, SHI Ding-Ji, XU Jie, XU Xu-Dong and Ru Bing-Gen   

Abstract: The cMT-like promoter (stat O-P) was used for the expression of mMT- Ⅰ cDNA in cyanobacterium, Anabaena sp. PCC 7120, to enhance its metal-binding ability and specificity. Shuttle vector pKT-MRE was constructed and replicated in E. coli HB101, and triparental conjugate transfer was applied for transforming cyanobacterial cells. Sm-screening, Southern and Western blotting analysis were used for the identification of the transgenic cyanobacterium clones. Transgenic cyanobacterial metal-absorption ability, heavy metal-resistance and photosynthesis measurements in the medium containing heavy metal ion indicated that the expression of foreign mMT- I enhanced the cyanobactefial heavy metal-resistance to 1.5 times in the transgenic Anabaena sp. PCC 7120.

Key words: mMT-Ⅰ, smt O-P, Transgenic filamentous cyanobacterium, Heavy metal-resistance, Anabaena sp. PCC 7120

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