J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (3): -.

• Research Articles •    

Isozyme Analysis of F 5 and BC 1F 4 from Cultivated Barley (Hordeum vulgare)× Roegneria ciliaris

LI Wan-Ji,LI Yi-Ping and LIU Fang   

Abstract: 栽培大麦,纤毛鹅观草,属间杂种,酯酶,过氧化物酶 ISOZYME ANALYSIS OF F5 AND BCiF4 FROM CULTIVATED BARLEY ( HORDEUM VULGARE ) ~ ROEGNERIA CILIARIS LI Wan-Ji LI Yi-Ping L1U Fang Abstract Esterase and peroxidase isozymes were analysed in the variants including 4 types, 16 lines of Fs, BC1F4 and the parents derived from cultivated barley ( Hordeum vulgare cv. Arupo) x Roegneria ciliaris (Trin.) Nevski in young roots, shoots, spikes and seeds. The zymogram patterns of esterase and peroxidase demonstrated that the 16 lines of F5 and BC1F4 had all or most bands of the cultivated barley parent cv. "Arupo", 1 to 3 bands from the male R. ciliaris, and new hybrid isozyme bands in various amount. Some bands of parent "Arupo" were lost. It suggested that the genetic substances come from R. ciliaris were stably inherited to the progenies of selfing and backcrossing, and there were some variations among the lines. There was certain relationship between isozyme variance and plant characters. Thus, in identifying the translocation lines by isozyme analysis, it would be preferable to study the various organ-specific isozymes or to trace one type of isozyme pattern in consequence.

Key words: Cultivated barley, Roegneria ciliaris, Intergeneric hybrid, Esterase, Peroxidase

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